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Added On 2011/11/15 7:09:27 PM Kannada. Added On 2011/10/18 11:55:19 AM Tamil. Added On 2011/07/23 3:01:01 AM.Purification of gamma-glutamylcysteine synthetase, gamma-glutamylcysteine synthetase b and o-acetylserine(thiol)lyase from a recombinant Escherichia coli strain.

A Escherichia coli strain that contained a plasmid having the genes encoding gamma-glutamylcysteine synthetase (EC 6.3.2.2), gamma-glutamylcysteine synthetase b (EC 6.3.2.2) and o-acetylserine(thiol)lyase (EC 4.1.3.15) was established. The resulting cells were disrupted and crude extracts were subjected to fractionation by polyethylene glycol fractionation and anion exchange chromatography to obtain pure gamma-glutamylcysteine synthetase, gamma-glutamylcysteine synthetase b and o-acetylserine(thiol)lyase.Underlying disease and associated factors in children with secondary amebic meningoencephalitis in Santiago, Chile.

A case-control study was conducted in the Microbiology Laboratory of Cemara Hospital, Santiago, Chile, to estimate the prevalence of underlying diseases and associated factors in a group of children with secondary amebic meningoencephalitis (SAM) treated at the hospital between 2001 and 2003. One hundred twenty-four children with SAM were enrolled in the study. Sixty-four (51.6%) were male and the mean age was 8.3 years (SD 5.1 years). Sixty-three (50.4%) subjects had had a previous hospitalization for SAM, usually within the previous 2 years. Of the 124 children, 47 (38.5%) had undergone previous surgery, 36 (29.5%) had chronic otitis media and 33 (26.6%) had a prematurity record, while chronic suppurative otitis media was found in 14.5%. There were significant differences between children with and without prematurity in a 0b46394aab